Specter on 29/4/2013 at 03:16
Quote Posted by dethtoll
You're from ThiefGen aren't you? I was making a joke.
I know you weren't serious, but I thought you may not have noticed that I'm Canadian, or that Im not longer in ATC. I was more trying to be clear than I was trying to negate your joke. Also, Ive seen the term Thiefgen tossed around in a few places, and maybe its because Im not here often enough, but Im not sure what it refers to. If it just means the Thief portions of this website, then yes. If not, forgive my ignorance.
@Fett:Thanks for pointing out your thread to me. I have great respect for what you lived through and overcame, and for sharing your story afterward!
june gloom on 29/4/2013 at 05:23
Yes it refers to the Thief subforums, but also to the culture in that forum.
Aja on 29/4/2013 at 05:49
After a year of or so of job hunting while being a caterer/hipster barista I managed to get a job at the provincial government. I'm a copy editor for Hansard, which is the branch that produces the official transcripts of the Legislative Assembly of Alberta. I started out as a transcriber but was recently promoted. I get to listen to MLAs all day droning and babbling and being generally idiotic, and then I have to make sure that what they say ends up in the official record adhering to our arbitrary and amazingly specific style rules. There's both a certain satisfaction and futility to it, but I enjoy it well enough. Bending all their incomplete sentences into something readable can be an entertaining challenge at times, though a horrible frustration at others.
Mostly I enjoy that it gives me time to work with my band, which is my true passion. We're aiming for an album by the summer, and it's mostly all I can think about at this point. I hope to be able to someday do that for a living above all else.
Volitions Advocate on 29/4/2013 at 07:00
Aja, I'm moving to Red Deer next month to start a new career and My long time band-mate lives in Morinville. We're planning on getting something going since I'm moving into such close proximity. Maybe at some point we could play a show together. Could be fun.
On that note, I finished my B.Music this last Christmas majoring in digital audio arts. So I'm kind of a recording engineer / gadget maker / electonics troubleshooter (so long as it involves music) / software engineer (again... so long as it involves music) / Composer / whatever else. Very tied into the whole Audio/Visual realm but with a severe leaning toward the audio and music side of things.
I have just finished as a Teaching assistant at the University of Lethbridge, worked 2 jobs as a Security Guard as a mobile patrolman / ATM babysitter and in a Hospital, and finishing up this week as a warehouse technician for Pepsico working at a Frito Lay plant. (i worked all 4 of these jobs simultaneously for the last 4 months too :S )
But i'm leaving all that for a job as a technician for an outdoor movie theatre company that sets up cinemas for Expo events, private rentals and drive in's and the like. They're grooming me to be their Operations manager for the province by end of year. Which means for me that I finally get something hands on, enjoyable, and related to my field of study to do, and only one job with time for my family, and one that hopefully pays enough to live comfortably while I pay both mountains of student debt I currently hold. (yep.... 2 mountains)
More time with the family is going to be important too, since tomorrow morning (in about 6 hours actually) we're heading to the hospital to have our 2nd baby.
faetal on 29/4/2013 at 09:26
Quote Posted by Specter
@Faetal: Thanks for pointing out the other thread!
No worries! I wouldn't worry about starting a new one either :)
Quote:
As for your research, I have a very limited science background; does in vitro alternatives mean you would use something like tissue samples rather than a live animal?
Using cells lines mostly, though it could feasibly just be done by modifying protein isolates if all you are looking at is e.g. modification of proteins by chemicals, though if you get too removed from biological systems, the phrase tends to be
in chemico. Working with tissue isn't feasible since it would have to come from living things, which we're looking to avoid. I did set up my original comparisons between cell lines derived from human skin and
ex vivo (meaning tissue explants from living things) human skin from mastectomy operations.
Anyway, the point is that from this year, it is illegal to sell or market any cosmetic or toiletry product in the EU which contains anything which has been tested on animals*. I'm partly funded by Unilever, because if they want to design a new range of shampoo or surface cleaner or some shit, any chemicals they use (or even untested combinations of known safe chemicals) will need to be screened using comprehensive non-animal screening methods, which don't yet exist. Hence all of the affected industries are panicking.
I hope you managed to stay awake for all of that :)
* - as in, tested on animals since the new legislation, not anything which has ever been tested on animals.
Vivian on 29/4/2013 at 09:41
As far as I recall/know (correct me if wrong, I've always wondered)
In Vitro (in glass) = experiments on tissues (in glass as in in a testtube or petri dish or whatever)
In Vivo (in life) = experiments on intact, functional organisms
Ex Vivo (after life) = experiments on intact, dead organisms or parts of organisms (limbs, organs, etc)
Are there any more? Basically, I'm asking because I do quite of a lot of work that pretty much amounts to flapping disembodied limbs around (we place intact limbs in loading jigs [a steel frame with a car-jack at one end] and see how they deform under loads with no activation of muscles [because it's just a leg]. Idea is to find out what the passive, spring-like stiffness and dashpot-like damping characteristics of the limb are). I've called that Ex Vivo in the past, but you seem to use that phrase differently, faetal.
faetal on 29/4/2013 at 10:02
Probably more than there needs to be:
In chemico: modelling any piece of biological process using inert chemicals as models for biomolecules.
In silico: computer modelling of biological (or chemical I guess) systems.
It reminds me of the whole -ome / -omics suffix being applied to an increasing list of ever more niche areas of biochemistry.
Genome, proteome, transcriptome, metabolome, secretome, inflammome, angiome.... The annoying part of that being that everything after proteome is just a sub-class of proteome, since I think it is only the protein crowd deciding they need nomenclature which makes their niche sound like an entire field. :/
I really fucking digress.
Kolya on 29/4/2013 at 12:08
Quote Posted by dethtoll
What are you even on about?
It was a compliment as dema assumed. I AM A GODDAMN PIXEL PUSHER MYSELF. See my post on the first page of this thread.
I therefore have a deep and slightly jealous admiration for anyone who creates things that aren't gone with a power outage.
DDL on 29/4/2013 at 12:19
Quote Posted by faetal
Genome, proteome, transcriptome, metabolome, secretome, inflammome, angiome.... The annoying part of that being that everything after proteome is just a sub-class of proteome, since I think it is only the protein crowd deciding they need nomenclature which makes their niche sound like an entire field. :/
Hey! Transcriptome isn't a subclass of proteome, and neither is the metabolome, generally speaking. :P
Most of the secretome etc bullshit is, admittedly (though even then you have secretory/inflammatory/angiogenic molecules that are non-protein based).
Or am I misreading your use of "everything after" and just being a massive pedant?*
*I am this anyway, admittedly
faetal on 29/4/2013 at 13:57
It was just lazy short-hand on my part.
It is complex when you account for e.g. a mix of lipids, nucleic acids and proteins, but I kind of wish it didn't have to be a new -omics every time a process was covered by more than 10 papers after 2008. It gets a bit meaningless.
